Maosheng Xia
The First Hospital of China Medical University, China
Title: Leptin improves the locomotion recovery of spinal cord injury
Biography
Biography: Maosheng Xia
Abstract
Spinal cord injury (SCI) causes long-term disability and has no eff ective clinically treatment. Th e initial trauma always results in permanent functional impairment and severe disability followed by secondary injury mechanism, which is characterized by increased infl ammation, glial scarring and neuronal cell death. Leptin (a glycoprotein) could induce the activation of Janus kinase (JAK2)/signal transducers and activators of transcription-3 (Stat3) pathway via leptin receptor. In vivo, we discovered the intraperitoneal injection of leptin improved the locomotion recovery of spinal cord injury. Th en, we researched the neuro-protective and anti-infl ammatory role of leptin on the spinal cord neurons and astrocytes. In the cultured neurons, we discovered leptin administration could enhance the expression of caveolin-1, block the composition of P2X7R-Panx1 complex and reduce the damage to neurons induced by ATP or by modeling operation of SCI. Even without injury operation, the pretreatment with leptin could suppress neuronal Ca2+ imaging triggered by ATP in spinal cord of live transgenic mice. In the cultured astrocytes, we discovered that: 1) the chronic administration of leptin could suppress the release of AA and PGE2 stimulated by ATP from the cultured spinal cord astrocytes; 2) leptin could elevate the expression of caveolin-1 through JAK2/Stat3 signaling pathway; 3) the increased caveolin-1 blocked the conjunction between Src and EGFR; 4) our results highlight leptin as a promising therapeutic agent for SCI.